Fig. 1: UVR8 structure, emission spectra and quantum yields.
From: Dynamics and mechanism of dimer dissociation of photoreceptor UVR8
a Overall structure of UVR8 homodimer with two tryptophan pyramid centers (orange), interfacial acidic residues (pink), interfacial basic residues (blue) and water molecules (light blue) highlighted. b Local structure around the tryptophan pyramid center. Two critical salt bridges, R286-D96/D107 and R338-E43/D44, are near W285. D129 and R234 are close to W233. D129 is hydrogen-bonded to W233 indole ring. c Steady-state emission spectra of WT, single Trp mutants and W285F/D129N are shown in various colored lines. The emission of free Trp in buffer was also showed in a dashed line for comparison. The inset shows fluorescence quantum yields (QF) of WT, free Trp and selected mutants with the standard deviation of five measurements. d Dissociation kinetics and total quantum yields (QY) of various UVR8 samples with the standard deviation of five (wild type) and three (all others) measurements.
