Fig. 5: Influences on transcripts involved in hepatic cholesterol and bile acid metabolism in gallstone-resistant mice carrying Desulfovibrionales. | Nature Communications

Fig. 5: Influences on transcripts involved in hepatic cholesterol and bile acid metabolism in gallstone-resistant mice carrying Desulfovibrionales.

From: Gut microbiota promotes cholesterol gallstone formation by modulating bile acid composition and biliary cholesterol secretion

Fig. 5

A Heatmap shows mRNA expression levels of genes involved in hepatic bile acid and cholesterol metabolism from RNA-seq data in liver samples from C57BL/6J, AKR/J, and co-housing mice (n = 5 mice/group). “C”: C57BL/6J, “A”: AKR/J, “coH.C”: C57BL/6J co-housing with AKR/J and “coH.A”: AKR/J co-housing with C57BL/6J. B mRNA expression levels of key enzymes in hepatic bile acid synthesis and secretion, cholesterol synthesis, absorption, and secretion determined by quantitative real-time PCR (n = 5 mice/group). Data are expressed as mean ± SEM. Statistics was performed by two-sided ANOVA with LSD post hoc analysis between groups. C Quantitative real-time PCR analysis of mRNA expression of genes in hepatic bile acid and cholesterol metabolism in mice receiving fecal microbiota transplantation (FMT) from gallstone patient (FMT.GS, n = 7) or gallstone-free controls (FMT.GSF, n = 9). Data are expressed as mean ± SEM. Statistics was performed by a two-sided t test. Source data are provided as a Source data file.

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