Fig. 2: In situ bacterial replication rates across subsurface ecosystems ordered by ecosystem depth.

The figure depicts a beeswarm plot of iRep values of genomes (x-axis) across ecosystems (y-axis) with genomes colored according to their predicted metabolic potential and the black dot representing the median iRep value (individual iRep values in Supplementary Data 2). C represents carbon, N₂ nitrogen, H₂ hydrogen, O₂ oxygen, and S sulfur. Colored squares depict the sample type. Samples impacted by geological degassing and a sediment sample along with the respective aquifer sample are plotted separately. The top y-axis shows the sampling depth of the different ecosystems (Supplementary Table 5). In total, 895 genomes were used for this analysis with ≧ 70% completeness and ≦ 10% contamination based on 51 bacterial and 38 archaeal single-copy genes. The order of samples is given in Supplementary Table 5. p-Values are derived from two-sided student’s t-tests. The exact p-values from top to bottom are p < 2.2 × 10¹⁶ (minimal value in R) and p = 0.0003934, respectively.