Fig. 5: Mapping of the spatial and genetic distances of single-cell clusters in primary tumors from six IMPC patients.

a–f Genetic distance and spatial distance mapping tree of primary IMPC (P14, P16, P18, P19, P22, and P23). The spatial distance was obtained by establishing a coordinate system. The genetic distance was calculated by comparing the number of CNVs. The genomic tree is located on the left, and the different colors represent different clonal subpopulations. The spatial tree is located on the right, and the different colors represent the different regions. The mapping of cell cluster coordinates and genotypes was performed by minimizing the overlapping connections. g Heatmap of the copy-number profiles of single-cell clusters in P19. Unsupervised clustering based on CNV similarity between different single-cell clusters and the different evolutionary stages during which the cell clusters were isolated is indicated. The values on the right represent the copy number: ≤1 indicates a loss and ≥3 indicates a gain. h Tumor section pattern map of P19. A coordinate system for each slice was established to obtain the x-axis and y-axis information, and the spatial positional relationship of the cell clusters was extracted accordingly. The corresponding evolutionary period of the cell clusters is based on the clustering information.