Fig. 1: Hypocrates (NemR-cpYFP biosensor) design and spectral characteristics.
a The structure of NemRC106 (PDB ID: 4YZE) shows the N-terminal DNA-binding domain (colored blue), the C-terminal sensory domain (colored cyan), Cys106 and Lys175 (colored yellow), and the flexible loop (colored red) into which cpYFP was inserted. The N- and C-termini are indicated with N and C, respectively. b The proposed simplified scheme of NemR-cpYFP biosensors functioning in living cells. c The structure of Hypocrates is presented with NemRC106 colored blue/cyan, cpYFP colored yellow, the linkers between NemRC106 and cpYFP colored green, and the flexible loop colored red. The upper numbers represent amino acid numbering corresponding to the intact NemRC106, while the lower numbers represent numbering corresponding to the biosensor. d Optical properties of purified Hypocrates protein in PBS. e Hypocrates fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. f Purified Hypocrates (0.5 µM) fluorescence excitation spectrum behavior in the presence of NaOCl in saturating concentration. g Purified Hypocrates (0.5 µM) fluorescence excitation spectrum behavior in the presence of N-chlorotaurine (NCT) in saturating concentration. h HypocratesCS fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. i HypocratesKA fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. Source data are provided as a Source Data file.
