Fig. 5: Zn2+ and BMP-2 peptide co-modified TiO2 surfaces enhance osteogenic differentiation in vitro. | Nature Communications

Fig. 5: Zn2+ and BMP-2 peptide co-modified TiO2 surfaces enhance osteogenic differentiation in vitro.

From: Engineering immunomodulatory and osteoinductive implant surfaces via mussel adhesion-mediated ion coordination and molecular clicking

Fig. 5

A The illustration of experimental design; B, E ALP staining and (C, G) ARS staining of BM-MSCs cultured in osteogenic medium supplemented with MCM (B–C scar bar = 50 μm, three independent experiments); F ALP activity assay of the BM-MSCs on the different surfaces; D Images of the BM-MSCs after immunofluorescent staining:(green: OPN; red: cytoskeleton and blue: nuclei) (scar bar = 30 μm, three independent experiments) and (H) quantitative results; I–L Osteogenesis-related genes expression of the BM-MSCs cultured in MCM detected by RT-PCR (Alp, Runx2, Col1a1 and Opn). (E–L n = 3 biologically independent samples per group, by a one-way ANOVA with a Tukey’s post hoc test for multiple comparisons). Data are reported as mean ± SD, *p < 0.05, **p < 0.01. Exact P values were given in the Source Data file.

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