Fig. 4: Characterization of intron library across multiple sites in oRiboT.
From: Targeted editing and evolution of engineered ribosomes in vivo by filtered editing
a, b Additional group I self-splicing introns were tested for expression of oGFP when introduced into oRiboT at sites 1 or 2. c–e Fluorescence of oGFP-oRiboT variants with intron candidates at site 1 and Tetrahymena intron at site 2, 3, or 4. f RT-PCR was performed on total purified RNA from cells expressing oRiboT with Tfa, Tfb, or Tfc intron at site 1 or site 2 to determine whether introns were completely spliced out of oRiboT and ligated post-splicing. Intron abbreviations correspond to Supplementary Table 5; +C = oRiboT with no intron; −C = no oRiboT plasmid. Values and error bars represent the mean and standard deviation of n = 3 biologically independent replicates.
