Fig. 4: Increased MMP-12 levels in WT and Trpml3^−/−.

a Quantification of the levels of different chemokines/cytokines and MMPs in BALF isolated from 4-month old WT and Trpml3^−/− mice using Multiplex analysis. b Repeated Multiplex analysis of MMP-12 levels in BALF. c, d MMP-12 quantification in BALF isolated from 4-month old WT and Trpml3^−/− mice using ELISA. One single dot corresponds to BALF from one mouse, each in c, d. e, f qRT-PCR data showing mRNA expression levels of Mmp-12 in AMΦ (WT and Trpml3^−/−). g Quantification of total cell numbers in BALF using the CASY1 cell counter. h Quantification of cell numbers in BALF using morphological criteria on May-Grünwald-Giemsa-stained cytospins. i MMP-12 quantification in the supernatant of cultured AMΦ isolated from 4-month old WT and Trpml3^−/− mice using ELISA. One single dot corresponds to the AMΦ SN from one well, each. Statistical analysis of datasets a-i was performed by using Student’s t test, unpaired, two-tailed (**p < 0.01, ****p < 0.0001). j, k MMP quantification in the supernatant of cultured AMΦ isolated from 4-month old WT and Trpml3^−/− mice using Multiplex and ELISA. One single dot corresponds to the AMΦ SN from one well, each. Two-way ANOVA followed by Tukey’s post hoc test; ***p < 0.001 (j) or Student’s t test, unpaired, two-tailed; **p < 0.01 (k). l Desmosine ELISA of BALF isolated from WT and Trpml3^−/− mice. One single dot corresponds to BALF from one mouse. Student’s t test, unpaired, two-tailed; **p < 0.01. m Verhoeff-Van Gieson (VVG) staining of formalin-fixed, paraffin-embedded lung sections of female, 4-month old WT or Trpml3^−/− mice (Mcoln3^tm.1.1Jga) treated either with PBS or porcine pancreatic elastase. Elastic fibers are stained blue-black, collagen appears red, and other tissue elements yellow. Scale bar 100 µm. n Quantification of elastin fibers as counts per field in VVG stained lung tissue sections from 6–8 mice per group. One dot corresponds to the mean count of elastin fibers in 8-10 fields of view per mouse lung. *p < 0.05, ****p < 0.0001; One-way ANOVA followed by Tukey’s post hoc test. In all figures, each single dot corresponds to one biologically independent sample. Data are mean ± SEM. Source data are provided as a Source Data file.