Fig. 3: Mutagenesis in BRCA1 deficient cells is regulated by 53BP1. | Nature Communications

Fig. 3: Mutagenesis in BRCA1 deficient cells is regulated by 53BP1.

From: BRCA1 deficiency specific base substitution mutagenesis is dependent on translesion synthesis and regulated by 53BP1

Fig. 3

a-c The mean number of newly arising SBS (single base substitution, a), insertion (b) and deletion (c) mutations detected in DT40 cell clones of the indicated genotypes following 50 days of culturing an ancestral clone (mock treatment). d–e The mean number of newly arising SBS (d), insertion (e) and deletion (f) mutations detected in DT40 cell clones of the indicated genotypes following 50 days of culturing that included four 1-h treatments at weekly intervals with 10 μM cisplatin. A sub-classification of deletion events according to size is shown by shading in (c, f). Error bars indicate SEM of the column totals, individual values are also shown with red symbols. The significance of tested differences is shown (ns not significant, unpaired two-sided t-test, data were derived from genome sequencing of biologically independent samples, see Supplementary Data 4 for sample sizes). Source data are provided as a Source Data file.

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