Fig. 2: Involvement of PRF1, GZMB, and FAS/FASLG in CTL-induced cell death of myoblasts and myotubes.
From: Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies
a The viability of H2KbOVA-myoblasts co-cultured with OT-I CTLs (n = 80), Prf1−/−OT-I CTLs (n = 59), or Gzmb−/− OT-I CTLs (n = 61). The viability was visualized by PI staining and assessed with time-lapse imaging. Data are presented as mean and SD. Two-way analysis of variance (ANOVA) test, followed by Dunnett’s test. *p < 0.05, **p < 0.01. b The viability of H2KbOVA-myotubes co-cultured with OT-I CTLs (n = 30) or Prf1−/− OT-I CTLs (n = 26). c The viability of H2KbOVA-myotubes co-cultured with OT-I CTLs (n = 42) or Gzmb−/− OT-I CTLs (n = 40). d The viability of H2KbOVA-myoblasts co-cultured with OT-I CTLs in the presence of FAS-Fc chimera protein (1 μM: n = 42, 10 μM: n = 50) or the control Fc protein (n = 48). Data are presented as mean and SD. e The viability of H2KbOVA-myotube co-cultured with OT-I CTLs in the presence of FAS-Fc chimera protein (0.1 μM: n = 57, 1 μM: n = 50, 10 μM: n = 44) or the control Fc protein (0.1 μM: n = 36, 1 μM: n = 33, 10 μM: n = 34). Representative data of three independent experiments are shown. Log-rank test, followed by Holm–Sidak multiple comparisons. **p < 0.01.
