Fig. 5: Cohesin expression and localization are unchanged by PARP inhibition. | Nature Communications

Fig. 5: Cohesin expression and localization are unchanged by PARP inhibition.

From: The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity

Fig. 5

A RT-qPCR of each cohesin component, each normalized to 18S, respectively, with and without olaparib treatment in LCL. Data displayed as fold change in expression of olaparib treated LCLs divided by control. The data presented is an average of three independent experiments. Source data are provided as a Source Data file. B Western blots of all cohesin components were independently collected from three biological replicates of control or 2.5 µM olaparib treated LCL after subcellular fractionation. Source data are provided as a Source Data file. C Immunoprecipitation (IP) of SMC3 component of cohesin in three biological replicates of LCLs, with or without PARP inhibition. Input lysate was collected before IP and ran along IP material for SMC3 and PARP1 western blots. Source data are provided as a Source Data file.

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