Fig. 3: Investigation of the stromal compartment of non-lactating and lactating mammary epithelial cells. | Nature Communications

Fig. 3: Investigation of the stromal compartment of non-lactating and lactating mammary epithelial cells.

From: Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk

Fig. 3

a Stromal cells were classified into vascular accessory (VA), endothelial (EN), fibroblasts (FB) and immune (IM) cells. b Canonical stromal markers were used to classify the different stromal subtypes where LMC only contained IM cells. c Sub-setting and re-clustering of IM cells revealed that both myeloid and lymphocytic lineages were sequenced from both NMC and LMCs. d CellChat analysis between luminal and immune cell subtypes identified in milk (i). ii Observed interactions between the cell types. iii All immune cell subtypes signal to LC1 via the EGF, MK and SPP1 signalling pathway (selected receptor–ligand pairs shown), whereas LC1 and LC2 luminal clusters from milk are signalling to milk immune cells via MHC-II, MHC-I, CSF and GRN signalling pathways (selected receptor–ligand pairs shown).

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