Fig. 7: Flow cytometric analysis of Tf and H-Ft binding to hTfR1 in the presence of ch128.1/IgG1. | Nature Communications

Fig. 7: Flow cytometric analysis of Tf and H-Ft binding to hTfR1 in the presence of ch128.1/IgG1.

From: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections

Fig. 7

Erythroblasts, which are known to express high levels of hTfR1, were differentiated and cultured from bone marrow CD34+ cells. The erythroblasts were incubated on ice with (a) a combination of Tf and an IgG1/κ isotype control or ch128.1/IgG1, (b) a combination of H-Ft and IgG1/κ isotype control or ch128.1/IgG1 or (c) a repeat of the H-Ft binding experiment that included the anti-hTfR1 antibody M-A712. Histograms represent the fluorescence intensity due to the binding of Tf or H-Ft to hTfR1 on the surface of the cells.

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