Fig. 9: Internalization of H-Ft into MM.1S cells in the presence of ch128.1/IgG1. | Nature Communications

Fig. 9: Internalization of H-Ft into MM.1S cells in the presence of ch128.1/IgG1.

From: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections

Fig. 9

a, b Standard and (c–e) imaging flow cytometric analysis of H-Ft internalization in the presence of the IgG1/κ isotype control (red line), ch128.1/IgG1 (green line) or the anti-hTfR1 antibody M-A712 (blue line). Negative control unstained cells (gray-filled) are also shown. a MM.1S cells incubated on ice for 60 min, after 30 min at 37 °C and after 60 min at 37 °C. b Median fluorescence intensities of H-Ft fluorescence over time. c Representative images of cells incubated for 60 min at 37 °C. d Internal fluorescence intensities of imaging flow cytometry analysis at 37 °C for 60 min. e Geometric mean fluorescence intensities of internalized H-Ft over time.

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