Fig. 1: Global changes in the NMR methylome during aging.

a Schematic of the study design suggesting changes of the NMR blood methylome during aging b Sample distribution of 107 NMR blood samples used in this study (one sample per animal). c Global DNAm level of all 3,089,098 CpG sites as a function of age. d Mean Shannon entropy of CpG levels (Mean entropy) for all NMRs (left panel) and NMRs older than 1 month (right panel). e Density plot of methylation levels for a representative NMR methylome in each year. f Proportion of methylation levels 0, 1 and ~0.5 for NMRs older than 1 month (Met 0 prop, Met 1 prop, Met 0.45-0.55 prop, respectively). g Histogram of Pearson correlation coefficients of methylation levels and ages for each CpG site in the NMR. h PCA of 758 CpG sites that are significant after Bonferroni correction i Mean methylation level of negatively correlated CpG sites (Mean DNAm BonfN) and positively correlated CpG sites (Mean DNAm BonfP). Number of samples (n), Pearson correlation coefficient (r), its two-sided p-value (p) are indicated for the specific panels. Source data are provided as a Source Data file.