Fig. 1: Development of ZFDs.
From: Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases
a ZFD (zinc finger deaminase) architecture. Split-DddAtox halves are fused to the C terminus of ZFPs (zinc finger proteins) (C type). b Optimization of the ZFD platform using pTarget libraries. pTarget plasmids contain a spacer region that ranges in size from 1–24 bp (shown in red) and ZFP DNA binding sites (shown in green). ZFD constructs contain AA (amino acid) linkers of different lengths (shown in yellow and orange) and different DddAtox split sites and orientations (shown in blue). c, d ZFD activities were measured at on-target sites in the pTarget library to examine the effect of the variables described in (b). ZFD pairs with linkers of the same (c) or different (d) lengths in the left and right ZFD were tested. Base editing frequencies were measured by targeted deep sequencing of the relevant region of pTarget plasmids. Data are shown as means from n = 2 biologically independent samples. Source data are provided as a Source Data file.
