Fig. 4: Activity of mitoZFDs, TALE-based DdCBEs, and ZFD/DdCBE hybrid pairs.
From: Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases
a DNA sequences of the binding regions of the mitoZFD and TALE-DdCBE pairs. Sites recognized by the TALE-DdCBEs are highlighted in green and for the mitoZFDs in blue. The upper sequence represents the mtDNA heavy strand and the lower sequence represents the mtDNA light strand. b Frequencies of cytosines edited by ZFDs, TALE-DdCBEs, and ZFD/DdCBE hybrid pairs. All statistical analysis for comparing with untreated samples was conducted using unpaired Student’s t-test (two-tailed) in GraphPad Prism 8. Statistical significance as compared with untreated samples was denoted with *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, n.s. (not significant) P > 0.05. Data are shown as means from n = 2 biologically independent samples. c Heat maps of base editing activities at each base position. The red box indicates the spacer region for each construct. The blue arrows indicate the position in the mtDNA. Source data are provided as a Source Data file.
