Fig. 3: Determination of the in vivo biochemical function of PECPS by transient expression of PECPS in N. benthamiana and knockdown expression of PECPS in A. sinensis calli. | Nature Communications

Fig. 3: Determination of the in vivo biochemical function of PECPS by transient expression of PECPS in N. benthamiana and knockdown expression of PECPS in A. sinensis calli.

From: Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood

Fig. 3

a MS chromatograms (EIC) of 5-hydroxy-1,7-bis(4-hydroxyphenyl)heptan-3-one (1R) and 2-(4-hydroxyphenylethyl)-4H-chromen-4-one (P1) accumulated in N. benthamiana leaves: (i) standard of P1; (ii) leaves infiltrated with benzoyl-CoA and Agrobacterium harboring pCAMBIA1300-35S-pecps; (iii) Leaves infiltrated with Agrobacterium harboring pCAMBIA1300-35S-pecps; (iv) leaves infiltrated with Agrobacterium harboring pCAMBIA1300-35S vector. b MS and MS2 spectra of 1R and P1. c MS chromatograms (EIC) of the major PECs 1–7 (structures could be found in Supplementary Fig. 22) accumulated in A. sinensis calli treated by different methods: (i) treated with 150 mM NaCl; (ii) knockdown PECPS expression and treated with 150 mM NaCl; (iii) healthy calli. d The relative contents (1,5-diphenylpentane-1,3-dione was implemented as internal standard, IS) of PECs 1–7 accumulated in different A. sinensis calli. Data represent the mean ± SD (n = 3). Statistical analysis was performed with unpaired two-tailed Student’s t-tests. Significance is shown with asterisks: ****p < 0.0001, ***p < 0.001, **p < 0.01. Exact p-values and source data are provided in the source data file.

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