Fig. 5: Analysis of degrons from multiple substrates. | Nature Communications

Fig. 5: Analysis of degrons from multiple substrates.

From: Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C

Fig. 5

a–c Dwell time distributions from SMOR analysis of representative movies with Cy5-labeled ySecurin D box peptide (a 1 nM), Clb5 D box peptide (b 1 nM), and Hsl1 KEN peptide (c 1 nM) binding to the indicated GFP-tagged proteins. d, e Analysis of hSecurin KEN box peptide (d 500 pM) or AurKB D box peptide (e 1 nM) binding to GFP-tagged human Cdh1 WD40 domain. Insets indicate mean dwell time ± SE (n indicates number of selected peaks). Results are representative of two independent experiments (Supplementary Table 1).

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