Fig. 6: Double degron substrate interactions. | Nature Communications

Fig. 6: Double degron substrate interactions.

From: Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C

Fig. 6

a Hsl1Halo fragment used in these experiments, showing sequences of KEN and D boxes. b–e Dwell time distributions from SMOR analysis of representative movies with Hsl1Halo carrying wild-type degrons and the indicated GFP-tagged binding proteins: b Cdh1WD40; c APC/CCdh1; d APC/CCdh1 with Apc10-4A mutations; e APC/CCdc20. f–g Analysis of Hsl1Halo with mutant D box, binding to: f Cdh1WD40; g APC/CCdh1. h–j Analysis of Hsl1Halo with mutant KEN, binding to: h Cdh1WD40; i APC/CCdh1; j APC/CCdh1 with Apc10-4A mutations. Insets indicate mean dwell time ± SE (n indicates number of selected peaks). Results are representative of two independent experiments (Supplementary Table 1).

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