Table 2 Dwell times for substrate interactions with human activators.

From: Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C

 

hCdh1WD40

hCdc20WD40

Hsl1 D box

4.52 s

1.08 s

Hsl1 D box mutant

No binding

No binding

ySecurin D box

SF

SF

Clb5 D box

SF

0.054 sa

Hsl1 KEN

1.48 s

SF

ySecurin KEN

0.869 s

SF

Acm1 ABBA

No binding

No binding

AurKB D box

0.202 s

SF

hSecurin KEN

0.174 s

No binding

  1. Dwell times are representative of two independent replicates, listed in Supplementary Table 1.
  2. SF single frame.
  3. aCalculated dwell time is less than three times the frame rate and is, therefore, less reliable.
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