Fig. 2: Transcriptomic analysis of purified Insr deficient β-cells.

a Workflow for RNA sequencing of FACS purified GFP-positive β-cells (100 per group) from 12-week old βInsr^KO and littermate control mice. b Insr and Igf1r mRNA levels from 100 GFP positive β-cells from individual βInsr^WT (female = black bar with red line, female = black bars with blue line), βInsr^HET (female = light pink bars, male = light blue bars) and βInsr^KO (female = dark red bars, male = dark blue bars) female (n = 4, 7, 8) and male (n = 5, 5, 4) mice. All samples were handled in a strictly blinded manner. Data were analysed by 2-way ANOVA with correction for multiple comparisons using Dunnett’s method. Data are presented as mean values +/− SEM. Source data are provided as Source Data file. c–f Genes expressed higher or lower in βInsr^KO vs. βInsr^WT are indicated by red and blue, respectively. c Volcano plot and significantly differentially genes across both sexes (n = see b). d Heatmap of all genes that were differentially expressed between any group, clustered by similarity. P values are shown on the right for the βInsr^WT to βInsr^KO comparison for all, males, and females (n = see b). e Differentially expressed genes between female βInsr^WT and βInsr^KO cells overlaid on a diagram of their predicted functional roles and subcellular locations. f Differentially expressed genes between male βInsr^WT and βInsr^KO cells overlaid on a diagram of their predicted functional roles and subcellular locations.