Fig. 4: Identification and importance of the oxyanion hole.
From: A structural basis for the diverse linkage specificities within the ZUFSP deubiquitinase family
a Superposition of ZUP1 (PDB:6EI1), TcZUP, and Mug105 active sites. The catalytic core domain is shown in cartoon representation and colored light gray. The catalytic triads are shown as sticks and colored orange. Ser-528 of TcZUP (brown) is in a suitable position to stabilize the tetrahedral reaction intermediate. The corresponding residues Gly-514 (ZUP1) and Asp-185 (Mug105) are shown as sticks and colored light gray and blue, respectively. b, c Activity of ZUP1148–578 (b) and TcZUP184–592 (c) oxyanion hole mutants against RLRGG-AMC. The RFU values shown are the means of triplicates. d, e Activity of ZUP1148–578 (d) and TcZUP184–592 (e) oxyanion hole mutants against K63-linked Ub6+ chains. f, g Activity of Mug105 oxyanion hole mutants against RLRGG-AMC (f) or K48-linked poly-ubiquitin chains (g). The RFU values shown are the means of triplicates. Source data are provided as a Source Data file.
