Fig. 6: Ubiquitin chain cleavage by AtZUP is dependent on UBZ.
From: A structural basis for the diverse linkage specificities within the ZUFSP deubiquitinase family
a–d Activity assays of FL AtZUP or UBZ-truncated AtZUP64–399 against K48-linked ubiquitin chains (a), K48-linked di-ubiquitin (b), RLRGG-AMC (c), and Ub-PA (d). Arrowheads mark the N- and C-terminal fragments of FL AtZUP (black) or AtZUP64–399 (green). e, f Activity assays of FL AtZUP and the internal truncations AtZUP∆219–275, AtZUP∆243–275 or AtZUP∆243–268 against K48-linked ubiquitin chains (e) or RLRGG-AMC (f). g Linkage specificity analysis with AtZUP∆219-275. A panel of di-ubiquitin chains was treated with AtZUP∆219–275 for the indicated time points. Source data are provided as a Source Data file.
