Fig. 1: Profiling of cell types in COPD using scRNAseq data.
From: Characterization of the COPD alveolar niche using single-cell RNA sequencing
A Overview of project design. Tissue from 17 lungs with advanced COPD and 15 control donor lungs were dissociated into single-cell suspensions. Individual cells were barcoded and sequenced for analysis. Similarly, lung tissue from 2 male (M) and 2 female (F) mice exposed to 10 months of cigarette smoke (CS) and 2 M and 2 F mice exposed only to room air (RA) were dissociated into single-cell suspensions, barcoded, and sequenced for analysis. B Uniform Manifold Approximation and Projection (UMAP) representation of 111,540 single cells grouped into 37 distinct cell types (left) with identification of COPD and control cells (right). Violin plots of normalized expression values for canonical cell-specific marker genes for C epithelial, D endothelial, and E stromal cells. AT1 alveolar epithelial type I cells, AT2 alveolar epithelial type II cells, PNEC pulmonary neuroendocrine cells, SMC smooth muscle cells, gCap general capillary, cMonocyte classical monocytes, ncMonocyte non-classical monocyte, Macs. macrophages, DC dendritic cells, cDC conventional dendritic cells, pDC plasmacytoid dendritic cells, NK natural killer cells, ILC innate lymphoid cells.
