Fig. 4: COPD endothelial cell types demonstrate universal and cell-type-specific transcriptional aberrations.
From: Characterization of the COPD alveolar niche using single-cell RNA sequencing
A UMAPs of all vascular endothelial (VE) and lymphatic endothelial cells from control and COPD subjects. UMAPs are color labelled by cell type (top) and disease status (bottom). B UpSet plot visualizing the properties of intersecting and unique sets of differentially expressed (DE) genes between COPD and control amongst endothelial (two-sided Wilcoxon rank-sum test, unadjusted P < 0.001, minimal fold change > 0.5). C Heatmap of corresponding differentially expressed genes between COPD and control amongst six subtypes of endothelial cells. Each column represents expression values for an individual cell. Columns are hierarchically ordered by endothelial subtype, disease phenotype, and then subject. Gray row (top): expression values for marker genes are unity normalized between 0 and 1 across all endothelial subtypes. Orange row (middle): z-scores of differentially expressed genes in three or more endothelial cell types between control and COPD. Yellow row (middle): z-scores of differentially expressed genes in both aerocyte and gCap cells between control and COPD. Blue and green row (bottom): z-scores of differentially expressed genes unique to aerocytes (blue) or gCap (green). Unity normalization and z-score calculations were performed using all endothelial subtypes.
