Fig. 1: Missing MZT1 modules bind on the outer surface of the recombinant human γ-TuRC.
From: Modular assembly of the principal microtubule nucleator γ-TuRC
a Two views on the cryo-EM reconstruction of the recombinant human γ-TuRC. Spokes are numbered and coloring is indicated. The positioning of all MZT1 modules is highlighted. The position of the MZT1 module on GCP3(14) was not visible in this structure due to substoichiometry and structural heterogeneity, but is resolved in other reconstructions at the indicated position (black circle). Substoichiometry of the γ-TuSC(1,2) and γ-TuSC(13,14) units in this reconstruction is indicated by grey coloring in the schematic representation. b Structure and composition of the lumenal bridge. Atomic model (PDB 6X0U) superposed to the segmented density of the lumenal bridge. Coloring as indicated. c Cryo-EM density of the MZT1-GCP3 module associated with GCP3(6). An atomic model of the MZT1-GCP3 module from the lumenal bridge (PDB 6X0U) was docked as a rigid body. MZT1-GCP3 modules directly interact with the adjacent γ-TuSC units via the GCP2 C-terminus.
