Fig. 3: A_2A receptor agonism ameliorated disease in a mouse model of lupus.

a Sera from the mice in Fig. 2a were analyzed for anti-RNA, anti-dsDNA, and anti-Smith antibodies by ELISA at 20 weeks of age. The graphs show relative absorbance values for the analyzed sera. Statistical significance was determined using a two-tailed unpaired t-test (left graph) or a Mann–Whitney test (middle and right graphs). b Spleens and LNs from the mice in Fig. 2a were analyzed at 20 weeks of age. The graph shows the number of splenocytes. Statistical significance was determined using a two-tailed unpaired t-test (p < 0.0001). c Kidneys from the mice in Fig. 2a were H&E-stained and were scored blindly for interstitial nephritis and glomerulonephritis at 20 weeks of age. Representative sections are shown (top: 1x, bottom: 20x). Lymphocyte and plasma cell aggregates are shown in the cortical medullary junction (red stars) and in the renal pelvis (blue stars). Patent (red arrows) and occluded capillary loops (black arrows) are shown. The graphs show the pathology scores. Statistical significance was determined using an ordinary two-way ANOVA with Sidak’s multiple comparisons test. Columns and error bars indicate the arithmetic mean and SD.