Fig. 1: LIN9 and LIN37 scaffold the MuvB complex. | Nature Communications

Fig. 1: LIN9 and LIN37 scaffold the MuvB complex.

From: The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes

Fig. 1

a Domain architecture of human LIN9 and LIN37, with regions of predicted and validated structure shown as blocks. The conserved LIN37 CRAW domain and LIN9 DIRP domain structures are determined here. MBD is the MYB-binding domain. b Schematic model for subunit interactions within MuvB. c The indicated tagged subunits or MuvB complex (GST-LIN52, Strep-RBAP48, His-LIN54504–749, GST-LIN37, and GST-LIN994–542) were expressed in Sf9 cells and extracts were precipitated with resin capturing the indicated tag. Proteins were visualized with coomassie staining. *Indicates impurities or degradation observed in some RBAP48 expressions. These bands are not pulled out from the tandem purification. The experiment was repeated three times with similar results. d HCT116 cells were transfected with plasmids encoding the indicated FLAG-tagged mouse protein. FLAG-tagged proteins were precipitated from extracts using anti-FLAG antibody and visualized with anti-FLAG immunoblotting and immunoblotting with antibodies that recognize RBAP48, LIN37, LIN54, and p130. A biological replicate of the experiment was performed, and results were similar.

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