Fig. 3: LIN37 CRAW domain binds both LIN9 and RBAP48. | Nature Communications

Fig. 3: LIN37 CRAW domain binds both LIN9 and RBAP48.

From: The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes

Fig. 3

a Interactions of LIN9 and LIN37 with the RBAP48 insertion loop. b Alignment of LIN37 sequences from organisms as in Fig. 2b. Residues that contact LIN9 (.), RBAP48 (*), and both LIN9 and RBAP48 (@) are indicated. c Close-up view of the LIN9-LIN37 interface. d The indicated FLAG-GFP control and FLAG-LIN9 WT and mutants were expressed by transient transfection in arrested HCT116 cells. Proteins were immunoprecipitated using anti-FLAG beads and the indicated proteins visualized by western blot. The 3x LIN9 mutant is E125A/W126A/F127A and the 4x LIN9 mutant is R174A/R175A/F180A/F181A. e Same as (d) but expressing the indicated RBAP46 and RBAP48 WT and mutant proteins. These immunoprecipitation experiments were performed each with a biological replicate, and results were similar.

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