Fig. 8: KP1 preserves the renal expression of endogenous Klotho after kidney injury.
From: A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signaling
a, b KP1 restored endogenous Klotho expression after UIRI. Representative Western blot (a) and quantitative data of Klotho (b) are shown. P value (from left to right): 0.007, 0.037 (one-way ANOVA with Fisher’s LSD test). n = 6 biologically independent animals. Data are presented as mean values ± SEM. c, d KP1 preserved endogenous Klotho protein after UUO. Representative Western blot (c) and quantitative data of Klotho (d) are shown. P value (from left to right): <0.001, <0.001 (one-way ANOVA with Fisher’s LSD test). n = 5 biologically independent animals). Data are presented as mean values ± SEM. e Immunohistochemical staining for Klotho protein in the obstructed kidney at 7 days after UUO. Arrows indicate positive staining. Scale bar, 50 µm. f Quantitative data of Klotho positive staining area in each high-power field. P value (from left to right): 0.001, 0.003. n = 5 biologically independent animals. Data are presented as mean values ± SEM. g, h Colocalization of Klotho and p-Smad3. Representative micrographs show Klotho localized in those tubules with nuclei negative for expression of p-Smad3. Nontumor kidney tissue from the patients who had renal cell carcinoma and underwent nephrectomy was used as normal controls (g). Sequential paraffin-embedded kidney sections from patients with membranous nephropathy (MN) were immunostained for Klotho and p-Smad3. Scale bar, 50 μm. Boxed areas are enlarged. h Scatter plots with linear regression show an inverse correlation between Klotho expression levels and p-Smad3 positive cells in human kidney sections. The Spearman correlation coefficient (r) and two side P value are shown. Source data are provided as a Source Data file.
