Fig. 6: Substrate scope and cascade reaction extension.

A Different substrates for Ti(Salen)-CALB cascade flow reactions. The system consists of 0.2 g Ti/SCs, 0.1 g CALB/SCs, 2.1 mL [BMIM]PF₆, 3 mL PEG-300, and 0.9 mL PBS (pH = 7.4, 0.05 M), 3 mL n-octane, 0.18 g emulsifier. Reaction conditions: aldehyde (0.05 M) and acetyl cyanide (0.2 M) in n-octane, 25 °C. a Flow rate from initial 0.6–0.3 mL h⁻¹ at the end. b Microreactors dosage is twice as much as a flow rate from 0.8 to 0.2 mL h⁻¹. c Microreactors dosage is three times as much as a flow rate from 0.5–0.2 mL h⁻¹. d Microreactors dosage is four times as much as (a), the flow rate from 0.8 to 0.2 mL h⁻¹. e Flow rate from 1.0 to 0.5 mL h⁻¹. f Flow rate from 0.8 to 0.3 mL h⁻¹. B Cascade reaction involving H-beta zeolite and CALB. g Cascade reaction for the synthesis of chiral 1-phenylethanol acetate. h Conversion and e.e. with time for the batch reaction using H-beta zeolite and CALB as catalysts. i Conversion and e.e. with time for the batch reaction using H-beta zeolite and CALB/SCs. j Conversion and e.e. with time for the flow reaction with H-beta zeolite and CALB/SCs co-localized in the biomimetic microreactor. k Specific activity of CALB in the batch or flow systems. The specific activity of CALB was calculated according to the conversion within the first 2 h for the batch reactions and after the conversion leveled off for the flow reactions. Reaction conditions are provided in Supplementary Information.