Fig. 9: Graphical representation of the in silico serotyping results for 3861 Shigella “routine” isolates according to each serotype prediction tool.

Stacked-bar chart displaying the results (percentage assignment) per tool for each Shigella serotype. E, the SeroPred tool implemented in EnteroBase18; T, ShigaTyper14; R, ShigEiFinder16 using short reads; F, ShigEiFinder using SPAdes assemblies. SB S. boydii, SD S. dysenteriae, SON S. sonnei, SF S. flexneri. A correct assignment means that the predicted serotype was the same as the phenotypically determined serotype. An uncertain assignment means that the correct serotype was listed among others or for ShigEiFinder that only the cluster level (Cs1 to Cs3) was obtained. An incorrect assignment means that the predicted serotype was different from the phenotypically determined serotype. “None” means that no serotype was predicted. More detailed results can be found in Supplementary Table 7.