Fig. 1: Lima1 expression pattern during mouse embryonic development. | Nature Communications

Fig. 1: Lima1 expression pattern during mouse embryonic development.

From: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism

Fig. 1

a E14.5 embryos sectioned and stained for E-cad, α-E-cat, N-cad, α-N-cat, β-cat or Lima1. Blue and magenta arrows indicate epithelial and muscle layers, respectively. b Normalized expression levels of Lima1 and E-cad during mouse pre-implantation development, based on Wang et al., 2004. c Oocytes and pre-implantation embryos stained for Lima1. Arrowheads indicate Lima1 localisation on the cortical cap in MII oocyte and in the polar body at the zygote stage. d Blastocyst stage embryos stained for Sox2 and E-cad or Sox2 and Lima1. e Morula and blastocyst stage embryos stained for AJ components, Lima1 and TE lineage markers (Gata3 or Cdx2). f Blastocyst and egg cylinder stage embryo stained for Lima1, Cdx2, Sox17 and DAPI. The PE is indicated by an arrowhead. Scale bars, (a), 1000 μm, upper panel and 100 μm, lower panels; (c), (d) and (e), 20 μm; (f), 10 μm. Experiments were repeated independently at least three times with similar results (a, c, d, e, f). Related to Figure S1.

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