Fig. 2: Lima1 expression in embryonic and extraembryonic stem cell lines.

a Western blot analysis of Lima1 expression in ESC and MEFs. b ESC and TSC stained for Lima1 and Oct4 (left panel) or E-cad and Eomes (right panel). c Schematic representation of ESC conversion to TSC-like cells. d Western blot analysis of Lima1 expression in ESC and TSC-like cells. e Western blot analysis of E-cad expression in ESC and TSC-like cells. f ESC and TSC-like cells stained for Oct4, Troma1 and Lima1. g Schematic representation of ESC conversion to EpiLC. h Western blot analysis of Lima1 expression in ESC and EpiLC. i Western blot analysis of E-cad expression in ESC and EpiLC. j Western blot analysis of β-cat expression in ESC and EpiLC. k ESC and EpiLC stained for Nanog (left panel) or E-cad and Lima1 (right panel). l Relative expression levels of Oct4, Nanog and Lima1 normalized to GAPDH in ESC and EpiLC. Mean values from three independent repetitions, data represent mean ± SEM, unpaired Student’s t-tests, 2-sided. m ChIP-seq peaks of pluripotency factors binding on the Lima1 locus in ESC. Polr2a peaks indicate the promoter region, ATAC-seq peaks indicate the accessible chromatin regions. Scale bars, (b), (f), (k), 50 μm. Experiments were repeated independently two times (a, b, e, f, i, j, k) or three times (d, h) with similar results. Related to Figure S2.