Fig. 4: Developmental potential of Lima1 KO ESC.
From: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism

a Teratomas derived from Lima1 KO or WT ESC. b Growth of WT and Lima1 KO teratomas. c Haematoxylin and eosin staining of sectioned teratomas. d Chimeric blastocysts containing control (WT) or Lima1 KO donor cells. e Quantification of integrated donor cells at blastocyst stage, data represent mean ± SEM, WT n = 6 embryos, Lima1 KO n = 6 embryos, unpaired Student’s t-test, 2-sided. f Chimeric E6.5 embryos containing control (WT) or Lima1 KO donor cells. g Quantification of the ectopic cells relative to the total epiblast cell number at E6.5. WT (n = 40 embryos), Lima1 KO (n = 32 embryos), three independent experiments. Data represent mean ± SEM, Mann–Whitney test, 2-sided. h Chimeric E13.5 embryos containing control (WT) or Lima1 KO donor cells. i Quantification of the ectopic cells relative to the total cell number of the foetus, determined by flow cytometry. WT (n = 7 embryos), Lima1 KO (n = 7 embryos), three independent repetitions. Data represent mean ± SEM, unpaired Student’s t-test, 2-sided. j Chimeric E13.5 embryos containing WT donor cells, stained for tdTomato, Podocalyxin and DAPI. k Chimeric E13.5 embryos containing Lima1 KO donor cells, stained for tdTomato, Podocalyxin and DAPI. Scale bars, (c) 50 μm; (d), 20 μm; (f) 50 μm; (h) 1000 μm; (j), (k),100 μm; Experiments were repeated independently three times (c, j, k) with similar results.