Fig. 6: Lima1 ectopic expression in primed pluripotent stem cells.
From: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism
a Control and Lima1-HA-expressing EpiLC stained for HA and DAPI. b Western blot analysis of Lima1 expression in control and Lima1-HA EpiSC. c Control and Lima1-HA-expressing conventional hiPSC stained for HA and DAPI. d Western blot analysis of Lima1 expression in control and Lima1 HA conventional hiPSC. e Principal component analysis of control EpiSC, Lima1-HA EpiSC, WT ESC and Lima1 KO ESC transcriptomes. f MA plot of gene expression in Lima1-HA vs. control EpiSC, two-tailed Wald-test with Benjamini–Hochberg correction for multiple testing and independent filtering of results. g Gene expression of naïve and primed pluripotency markers in WT ESC, Lima1 KO ESC, control EpiSC, Lima1-HA EpiSC. h Principal component analysis of control conventional hiPSC, Lima1-HA hiPSC and naïve hiPSC (Giulitti et al., 2019)50 transcriptomes. i MA plot of gene expression in Lima1-HA vs. control conventional hiPSC, two-tailed Wald-test with Benjamini–Hochberg correction for multiple testing and independent filtering of results. j Gene expression of naïve and primed pluripotency markers control conventional hiPSC, Lima1-HA hiPSC and naïve hiPSC. k Dissociated control and Lima1-HA-expressing EpiSC. l Dissociated control and Lima1-HA-expressing EpiSC treated with Y-27632. m Mean distribution of cell membrane fluctuation counts per cell over 90 s of time-lapse imaging with 5 s intervals from k and l; n > 177 cells for each condition, Mann–Whitney tests, 2-sided. n Dissociated naïve hiPSC cultured without or treated with Y-27632. o Dissociated control and Lima1-HA-expressing conventional hiPSC. p Dissociated control and Lima1-HA-expressing conventional hiPSC treated with Y-27632. q Mean distribution of cell membrane fluctuation counts per cell over 90 s of time-lapse imaging with 5 s intervals from n, o and p; n = 92 (Control hiPSC), 89 (Control hiPSC treated) 88 (naïve hiPSC), 100 (naïve hiPSC treated), 294 (Lima1-HA hiPSC), 245 (Lima1-HA hiPSC treated) cells; 2-way ANOVA with Tukey’s multiple comparisons. Scale bars, (a), (c), 50 μm; (k), (l), (n), (o), (p), 10 μm. Experiments were repeated independently two times (a, b, c, d) or three times (m, q) with similar results. Related to Fig. S5.
