Fig. 7: Ultrastructural analysis and expression of Lima1 deletion constructs in EpiSC.
From: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism
a Transmission electron microscopy of immunogold staining for the HA-tag in control and Lima-HA EpiSC. Arrowheads indicate the HA signal; mitochondria are marked in magenta. At least three technical replicates with similar results. b Transmission electron microscopy of double immunogold staining for Lima1 and Actin in control and Lima-HA EpiSC. Blue and red circles indicate the 15 nm and 10 nm gold particles, respectively; mitochondria are marked in magenta. At least three technical replicates with similar results. c Maps of the Lima1 constructs. d Western blot analysis of the expression of the Lima1 constructs detected by anti-HA antibody. e Mean distribution of cell membrane fluctuation counts per cell over 90 s of time-lapse imaging with 5 s intervals; n = 225 (Control), 245 (full-length Lima1-β), 68 (del LIM domain), 78 (del C-terminus), 51 (del N-terminus), 18 (E-cad-Lima1-β fusion) cells, one-way ANOVA. Scale bars, (a), (b) 1 μm. Related to Fig. S6.
