Fig. 8: Engraftment of Lima1-HA-expressing primed pluripotent cell in mouse pre-implantation embryos.
From: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism
a E3.5 and E4.5 chimeric blastocysts generated via morula aggregation using control or Lima1-HA-expressing EpiSC. b Quantification of the chimeric blastocysts containing control (22 embryos) or Lima1-HA-expressing (26 embryos) EpiSC at E4.5. Data represent mean ± SEM, n = 3 independent experiments, unpaired Student’s t-test, 2-sided. c Chimeric blastocysts generated via morula aggregation using control (upper panel) or Lima1-HA-expressing EpiSC (lower panel) and stained for Venus, Oct4 and DAPI. Arrowhead indicates integrated EpiSC. d E3.5 and E4.5 chimeric blastocysts generated via morula aggregation using control or Lima1-HA-expressing conventional hiPSC. e Quantification of the chimeric blastocysts containing control (30 embryos) or Lima1-HA-expressing (33 embryos) conventional hiPSC at E4.5. Data represent mean ± SEM, n = 4 independent experiments, Mann–Whitney test, 2-sided. f Chimeric blastocysts generated via morula aggregation using control (upper panel) or Lima1-HA-expressing conventional hiPSC (lower panel) and stained for Venus, Oct4 and DAPI. Arrowhead indicates integrated hiPSC. Scale bars, (c), (f), 20 μm. Experiments were repeated independently three times (a, c, d) with similar results. Related to Fig. S7.
