Fig. 3: mvk-1 mutant plants show reduced mevalonic acid-5-phosphate (MVP) levels.
From: Activation of the plant mevalonate pathway by extracellular ATP
Exogenous application of MVP partially restores the altered mvk-1 mutant phenotype. a–c Box-and-whisker plots of intermediate compounds in MVA pathway showing their relative abundances. ColQ and mvk-1 mutant root extracts were analyzed with a high-resolution mass spectrometer (HRMS) Orbitrap Velos (Thermo Fisher Scientific) coupled to a Thermo Vanquish HPLC (High Pressure Liquid Chromatographer; Thermo Fisher Scientific). MVA, Mevalonic acid; MVP, Mevalonic acid-5-phosphate; IPP, isopentenyl diphosphate. Asterisks indicate significant differences between ColQ and mvk-1 (means ± SEM, n = 8 biological replicates, *P < 0.05, two-sided Student’s t test). Box-and-whisker plots show max and min, 25–75th percentiles (box), and median (center line). Experiment was repeated three times with similar results. d 16-day-old ColQ and mvk-1 mutant seedlings grown on medium without or with 100 µM mevalonic acid-5-phosphate (MVP). Scale bars: 0.5 cm. e Fresh second leaf weight of 16-day-old plants (n = 21 leaves for ColQ and n = 24 leaves for mvk-1). Data represent mean ± SEM from independent experiments. Means with different letters are significantly different (P < 0.05). P values indicate significance relative to ColQ with mock treatment and were determined by one-sided ANOVA with multiple comparisons and adjusted using a Duncan post hoc test. Experiment was repeated three times with similar results.
