Fig. 1: etfβ is a sterile mutant with a defective meiosis phenotype when grown in a paddy field.
From: Nitrogen nutrition contributes to plant fertility by affecting meiosis initiation
a Whole plants of wild type (WT) and etfβ at heading stage grown in a paddy field. b Fine mapping of ETFβ. Vertical lines, marker loci; Rec number of recombinant plants. c Phenotypic comparison of anthers of the same size between WT and etfβ in Hainan (top) and Beijing (bottom); anthers were stained with toluidine blue (TBO). The right three images of etfβ in in Hainan and Beijing were aligned in order of the phenotypic severity. The arrows indicate abnormal apoptosis in the center of locules. E epidermis, En endothecium, ML middle layer, MP mature pollen, PMC pollen mother cell, T tapetum. Scale bars, 5 µm. d Mitochondrial localization of ETFβ in rice protoplasts. Red indicates mitochondria, and green indicates transient expression of ETFβ fused to green fluorescent protein (GFP) in rice protoplasts. The yellow fluorescence in the merged image (bottom right) shows mitochondrial localization. Scale bars, 10 µm. e Expression of ETFβ in WT anthers determined by RNA in situ hybridization. Hybridization with the sense ETFβ transcript was performed as a negative control. AR archesporial cell, ISPC inner secondary parietal cell, OSPC outer secondary parietal cell; PPC primary parietal cell, PSC primary sporogenous cell, SC sporogenous cell. Scale bars, 5 µm.
