Fig. 2: Cholinergic signals promote HSC quiescence in endosteal BM niches.
From: Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
a Ratio of quiescent HSCs (G0:G1 phases of the cell cycle) in the endosteal or central BM of Gfra2−/− or WT mice (N = 6; P = 0.0006). b Representative flow cytometry diagrams showing ki67/Hoechst 33342 of lin− sca1+ ckit+ (LSK) CD48− CD150+ HSCs isolated from the endosteal or central BM of Gfra2−/− mice or control Gfra2+/− mice (N = 6). The frequencies of the gated populations are indicated. c Ratio of quiescent HSCs in the endosteal or central BM of Gfra2−/−, Adrb3−/−, compound Gfra2−/;Adrb3−/− or WT mice (N = 3; P = 0.006). a, c Each dot is a mouse. Data are mean of biological replicates ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. ANOVA and Bonferroni’s multiple comparisons test.
