Fig. 4: Cholinergic-deficient niche compromises HSC quiescence, leading to increased reconstitution of primary recipients. | Nature Communications

Fig. 4: Cholinergic-deficient niche compromises HSC quiescence, leading to increased reconstitution of primary recipients.

From: Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis

Fig. 4

a Scheme illustrating competitive HSC transplantation in primary recipients. 500 endosteal LSK CD48− CD150+ HSCs and 105 BM helper cells were i.v. injected into lethally irradiated congenic mice of indicated genotype. b–g Donor-derived haematopoietic chimerism (b), B cells (c), T cells (d), eosinophils (e), neutrophils (f) and monocytes (g) at specified time following primary transplantation of HSCs from donors and recipients of specified genotypes (Gfra2+/− > CD45.1, N = 5; Gfra2−/− > CD45.1, N = 6; CD45.1 > Gfra2−/−, N = 4; CD45.1 > Gfra2+/+, N = 4). Data are mean of biological replicates ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. ANOVA and Tukey’s multiple comparisons test.

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