Fig. 5: Reduced quiescence and self-renewing programme in endosteal HSCs from cholinergic-neural-deficient mice.

Gene sets enrichment analysis (GSEA) comparing endosteal and central lin⁻CD48⁻sca1⁺ckit⁺CD150⁺ HSCs of WT mice and Gfra2^−/− mice (N = 4 biological replicates). a Enrichment map showing upregulated pathways in central (compared with endosteal) BM HSCs. b Downregulation of RET target genes in HSCs from Gfra2^−/− mice. c Enriched Notch1-Myc target gene expression in endosteal WT HSCs. d Datasets from the Reactome, Pathway Interaction Database and Hallmark database as well as published HSC datasets were used for comparison. The normalised enrichment score (NES) of significantly enriched datasets (False discovery rate (FDR) < 0.1) is depicted as heatmap. Red, blue, and white colours indicate upregulation, downregulation, and no change, respectively.