Fig. 6: Reduced HSC self-renewal in a cholinergic-neural-deficient niche.
From: Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
a Scheme illustrating competitive serial HSC transplantations. 500 endosteal LSK CD48− CD150+ HSCs isolated from primary recipients were i.v. injected together with 105 BM helper cells into lethally irradiated secondary recipients of the same genotype as the primary recipient mice. b–g Donor-derived haematopoietic chimerism (b), B cells (c), T cells (d), monocytes (e), eosinophils (f) and neutrophils (g) and at specified time following secondary transplantation of mice with specified genotype (Gfra2+/− > CD45.1, N = 3; Gfra2−/− > CD45.1, N = 4; CD45.1 > Gfra2−/−, N = 4). Data are mean of biological replicates ± SEM. *P < 0.05, ***P < 0.001. ANOVA and Tukey’s multiple comparisons test.
