Fig. 7: Nicotine induces HSC quiescence after transplantation.
From: Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
a Scheme illustrating experimental design. Irradiated WT CD45.2 recipients were transplanted with 2 × 106 BM nucleated cells from CD45.1 mice and treated with 65 µg/ml nicotine or vehicle in their drinking water over 7 weeks. b, c Circulating donor-derived (CD45.1+) haematopoietic cells (b, P = 0.03) and white blood cells (c, P = 0.006,0.04) at specified time after transplantation (N = 3). d Donor-derived B cells (P = 0.007), T cells (P < 0.0001) and myeloid cells (P = 0.009) from mice treated with nicotine or vehicle at 7 weeks (N = 3). e Cell cycle profiles of donor-derived lin-sca1+ckit+ (LSK) CD34-flt3- HSCs isolated from the endosteal or central BM 7 weeks after transplantation and chronic treatment with nicotine or vehicle (N = 3;P = 0.005,0.04). (b–e) Data are mean of biological replicates ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. (b–e) ANOVA and Sidak’s comparisons.
