Fig. 4: 3D focus locking for super-resolution single-objective selective-plane illumination microscopy over cellular volumes.
From: Direct-laser writing for subnanometer focusing and single-molecule imaging
a Laser light is reflected off a 45° mirror selectively illuminating a single plane within the sample. Fluorescence is captured via the same objective. Focus locking onto different axial planes is performed by using the 3D fiducial. b Brightfield image of 3D fiducial structures (shown schematically in Fig. 2c), taken with a x10 objective, located within individual wells of the soSPIM imaging chambers (central column) (n = 7 chambers). Scale bar = 200 µm. c soSPIM optical sections showing the distribution of CD47 labeled with Alexa-Plus 647 in HEK293 cells at the indicated depths (n = 6). d Corresponding dSTORM images of CD47 at the depths shown in (c) (n = 6). Scale bar = 5 µm.
