Fig. 3: Obese mEVs cause obesity-associated islet inflammation and β cell abnormalities. | Nature Communications

Fig. 3: Obese mEVs cause obesity-associated islet inflammation and β cell abnormalities.

From: Accumulation of microbial DNAs promotes to islet inflammation and β cell abnormalities in obesity in mice

Fig. 3

a The glucose-stimulated insulin secretion (GSIS) levels of 5wks HFD WT and Vsig4−/− mice (n = 5 per group). Effects of obese mEV treatment on GSIS (b, n = 5 per group) and the expression of key genes associated with β cell functions (c, n = 4–5 per group) in NCD Vsig4−/− recipient mice. d The GSIS levels of NCD WT recipient mice after 4 weeks of obese mEV treatment (n = 7 per group). The GSIS and cellular insulin levels of either NCD WT (e, n = 4–6 per group) or NCD Vsig4−/− (f, n = 7-8 per group) islets after in vitro treatment with obese mEVs. Effects of obese mEVs on GSIS and cellular insulin content of healthy human islets (g, n = 8 per group) or islets derived from patients with obesity (h, n = 8 per group). All experiments were repeated at least twice with similar results. Data are presented as the mean ± SEM. P values are determined by unpaired two-sided Student’s t test (a–h).

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