Fig. 4: Complement component 3 (C3) facilitates the capture of mEVs by islet Vsig4+ macrophages. | Nature Communications

Fig. 4: Complement component 3 (C3) facilitates the capture of mEVs by islet Vsig4+ macrophages.

From: Accumulation of microbial DNAs promotes to islet inflammation and β cell abnormalities in obesity in mice

Fig. 4

a The intensity of PKH26 fluorescence within islets of NCD C3−/− mice after 24 h of intravenous injection with PKH26-labeled obese mEVs. Scale bar = 20 µm. Representative images are from three independent experiments. Effects of obese mEV treatment on islet Il1b abundance (b, n = 8 per group), islet macrophage population (c, n = 6 per group), GSIS (d, n = 8 per group), and insulin abundance (e, n = 8 per group) and the expression of key genes associated with insulin synthesis and secretion (f, n = 8 per group) in NCD C3−/− mice. All experiments were repeated at least twice with similar results. Data are presented as the mean ± SEM. P values are determined by unpaired two-sided Student’s t test (b–f).

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