Fig. 1: GRP78 is expressed on the cell surface on primary AML blasts and PDX samples. | Nature Communications

Fig. 1: GRP78 is expressed on the cell surface on primary AML blasts and PDX samples.

From: CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells

Fig. 1

a Violin plots of gene expression analysis by RNA seq comparing HSPA5 expression on normal cord blood CD34+ cells to the TARGET dataset AML samples (N = 159). b Violin plots of microarray data comparing normal control HPCs to the TCGA (N = 244) dataset AML samples. Statistical analysis: TARGET and TCGA datasets, normal controls (CD34+ cells/HPCs) vs AML; T-test with pairwise comparisons was used, p-value < 0.05. The properties of the box-plots are defined as follows; minima: lower whisker = smallest observation greater than or equal to lower hinge − 1.5 * IQR (IQR = interquartile range: the difference between the 75th and 25th percentiles), box lower hinge = 25% quantile, box middle = median, 50% quantile, box upper hinge = 75% quantile, maxima: upper whisker = largest observation less than or equal to upper hinge + 1.5 * IQR. c Flow cytometric analysis showing cell surface GRP78 expression on normal NT T cells and normal bone marrow (BM) CD34+ cells56 and AML cell lines (left panel). Mean Fluorescence Intensity (MFI) of mAb (10C3 Dylight488 Ab) compared with Peptide (Biotin-Ahx-CTVALPGGYVRVC) staining in AML cell lines and healthy controls (right panel). d Flow cytometric analysis of 14 primary AML samples showing cell surface GRP78 expression (de novo N = 6, relapsed N = 4, therapy related N = 4). e Flow cytometric analysis of 5 AML-PDX samples showing percentage of cell surface GRP78+ cells. Source data are provided as a Source data file.

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